RESUMEN
The use of biological agents offers a sustainable alternative to chemical control in managing plant diseases. In this study, Bacillus velezensis IFST-221 was isolated from the rhizosphere of a healthy maize plant amidst a population showing severe disease symptoms. The investigation demonstrated a broad-spectrum antagonistic activity of IFST-221 against eight species of pathogenic ascomycetes and oomycetes, suggesting its potential utility in combating plant diseases like maize ear rot and cotton Verticillium wilt. Additionally, our study unveiled that IFST-221 has demonstrated significant plant growth-promoting properties, particularly in maize, cotton, tomato, and broccoli seedlings. This growth promotion was linked to its ability to produce indole-3-acetic acid, nitrogen fixation, phosphate and potassium solubilization, and biofilm formation in laboratory conditions. A complete genome sequencing of IFST-221 yielded a genome size of 3.858â¯Mâ¯bp and a GC content of 46.71%. The genome analysis identified 3659 protein-coding genes, among which were nine secondary metabolite clusters with known antimicrobial properties. Additionally, three unknown compounds with potentially novel properties were also predicted from the genomic data. Genome mining also identified several key genes associated with plant growth regulation, colonization, and biofilm formation. These findings provide a compelling case for the application of B. velezensis IFST-221 in agricultural practices. The isolate's combined capabilities of plant growth promotion and antagonistic activity against common plant pathogens suggest its promise as an integrated biological agent in disease management and plant productivity enhancement.
RESUMEN
Reports on development of resilient wheat mutants to aphid infestation-causing heavy losses to wheat production in many parts of the world, are scanty. The present study aimed to identify genetic diversity of wheat mutants in terms of varying degree of resistance to aphid infestation which can help protect wheat crop, improve yields and enhance food security. Resistance response to aphid infestation was studied on newly developed 33 wheat mutants, developed through irradiating seed of an elite wheat cultivar "Punjab-11" with gamma radiations, during three normal growing seasons at two sites. Data on various traits including aphid count per plant, biochemical traits, physiological traits and grain yield was recorded. Meteorological data was also collected to unravel the impact of environmental conditions on aphid infestation on wheat plants. Minimum average aphid infestation was found on Pb-M-2725, Pb-M-2550, and Pb-M-2719 as compared to the wild type. High yielding mutants Pb-M-1323, Pb-M-59, and Pb-M-1272 supported the moderate aphid infestation. The prevailing temperature up to 25 °C showed positive correlation (0.25) with aphid count. Among biochemical traits, POD (0.34), TSP (0.33), TFA (0.324) exhibited a high positive correlation with aphid count. In addition, CAT (0.31), TSS (0.294), and proline content (0.293) also showed a positive correlation with aphid count. However, all physiological traits depicted negative correlation with aphid count, while, a very weak correlation (0.12) was found between mean aphid count and grain yield. In PCA biplots, the biochemical variables clustered together with aphid count, while physiological variables grouped with grain yield. Biochemical parameters contributed most, towards first dimension of the PCA (48.6%) as compared to the physiological variables (13%). The FAMD revealed that mutant lines were major contributor towards total variation; Pb-M-1027, Pb-M-1323, Pb-M-59 were found to be the most diverse lines. The PCA revealed that biochemical parameters played a significant role in explaining variations in aphid resistance, emphasizing their importance in aphid defense mechanisms. The identified mutants can be utilized by the international wheat community for getting insight into the molecular circuits of resistant mechanism against aphids as well as for designing new KASP markers. This study also highlights the importance of considering both genetic and environmental factors in the development of resilient wheat varieties and pave the way for further investigations into the molecular mechanisms underpinning aphid resistance in wheat.
RESUMEN
Fusarium head blight (FHB) is a global cereal disease caused by a complex of Fusarium species. Both Fusarium graminearum and F. asiaticum are the causal agents of FHB in China. F. asiaticum is the predominant species in the Middle-Lower Reaches of the Yangtze River (MLRYR) and southwest China. Therefore, detecting F. asiaticum in a timely manner is crucial for controlling the disease and preventing mycotoxins from entering the food chain. Here, we combined rapid genomic DNA extraction, recombinase polymerase amplification, Cas12a cleavage, and lateral flow detection techniques to develop a method for the rapid detection of F. asiaticum. The reaction conditions were optimized to provide a rapid, sensitive, and cost-effective method for F. asiaticum detection. The optimized method demonstrated exceptional specificity in detecting F. asiaticum while not detecting any of the 14 other Fusarium strains and 3 non-Fusarium species. Additionally, it could detect F. asiaticum DNA at concentrations as low as 20 ag/µL, allowing for the diagnosis of F. asiaticum infection in maize and wheat kernels even after 3 days of inoculation. The developed assay will provide an efficient and robust detection platform to accelerate plant pathogen detection.
